Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil
نویسندگان
چکیده
منابع مشابه
Detection of non-enterotoxigenic and enterotoxigenic Bacteroides fragilis in stool samples from children in São Paulo, Brazil.
Non-enterotoxigenic bacteria of the Bacteroides fragilis group and enterotoxigenic B. fragilis were identified from children with and without aqueous acute diarrhea. In this study, 170 stool samples from 96 children with and 74 without diarrhea were analyzed. Enterotoxin production and the toxin gene detection were detected by cytotoxicity assay on HT-29/C1 cells and by PCR, respectively. B. fr...
متن کاملRapid detection of enterotoxigenic Bacteroides fragilis in diarrhoeal faecal samples.
BACKGROUND & OBJECTIVES Enterotoxigenic Bacteroides fragilis (ETBF) is an important aetiological agent of acute diarrhoeal illness in humans. It is a fastidious anaerobe and takes 3-5 days when grown in culture, and test for enterotoxin detection requires additional time conventionally. The present study was aimed to standardize a rapid and sensitive diagnostic assay and to screen the presence ...
متن کاملEnterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbiota of children
Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, base...
متن کاملDetection of enterotoxigenic Bacteroides fragilis by PCR.
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
متن کاملEnterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil.
The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Forty-nine strains (44.9%) exhibited the NTBF pattern III which possesses t...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 2003
ISSN: 0036-4665
DOI: 10.1590/s0036-46652003000400010